- - AGRICULTURE CORE CURRICULUM - - (CLF7000) Advanced Core Cluster: PLANT AND SOIL SCIENCE (CLF7350) Unit Title: PLANT PATHOGENS AND PESTS ____________________________________________________________________________ (CLF7352) Topic: PLANT PATHOLOGY Time Taught in Year(s) 3 hours 3 and 4 ____________________________________________________________________________ Topic Objectives: Upon completion of this lesson the student will be able to: Learning Outcome #: (F-2) - Discuss common diseases in your area. (F-2) - List possible ways that diseases are spread. (F-3) - Summarize the relationship of proper plant nutrition with improved plant health. (F-4) - Explain the effect of diseases on development, growth, and production of plants. (F-14) - Describe a nematode of your choice, the type of damage it does and the plants it infests. (F-17) - Differentiate between bacteria, fungi, virus and nematodes. (F-2) - Identify vectors that spread plant disease. Special Materials and Equipment: Rotted (soft) fruit samples. References: Agrios. PLANT PATHOLOGY. Page 7, 172-191, 435-446, 549-580, 612-623. Evaluation: Quiz by instructor TOPIC PRESENTATION: PLANT PATHOLOGY INTRODUCTION A. Plant diseases are important to man, because they cause damage to plants and plant products. B. The yield and quality of plants are reduced by a wide array of plant diseases. 1. A plant disease is the complex of symptoms caused by a pathogen on a plant. C. A plant pathologist is a person who studies plant diseases and works to diagnose and control them. D. Some plant diseases are easily controlled by one or another method. E. For other plant diseases, however the cost of control is as high or higher than the expected value of the crop. PLANT PATHOLOGY A. There are many ways in which plant disease pathogens can affect plants. 1. They can suppress the chlorophyll content. 2. They can reduce the leaf area. 3. They can curb the movement of solutes and water through the stems. 4. They sometimes reduce the water-absorbing capacity of the roots. 5. They suppress the translocation of photosynthates away from the leaves. 6. They sometimes promote wasteful use of the products of photosynthesis as in the formation of galls. B. Most pathogens are parasitic - they invade the host and obtain food from it. 1. Many are submicroscopic, making identification difficult. 2. By definition, plant pathogens are capable of spreading from one host to another. 3. The most important plant pathogens are bacteria, fungi, viruses, and nematodes. 4. Most of the plant pathogens have a saprophytic (living in dead or decaying organic matter) existence apart from their host plants during most of the year. C. Lets take a closer look at each type of pathogen. 1. Bacteria are small, single celled, microscopic organisms. a. Of the 1600 known bacterial species about 200 have been found to cause plant disease. b. Most plant infecting bacteria are rod-shaped and most have thread-like structures (Flagella) that propel them through liquids. c. Anything that moves and comes in contact with bacteria may spread them to other areas. 1) This includes farm equipment, rain, plant material, seeds, birds, insects, nematodes, and people. 2) To start an infection in a plant they must enter a natural opening or a wound. d. Bacteria are significant as pathogens because of their ability to multiply rapidly. 1) They divide by binary fission. (One bacterium divides in half and becomes two bacteria.) 2) Under proper environmental conditions this division takes place every twenty minutes. 3) At this rate 1 bacterium can give rise to 17 million cells within 12 hours if food, moisture, and temperature are favorable. e. Bacterial diseases can be seen as galls, leaf spots, soft rots, scabs and systemic disorders. f. Controls for bacterial infections include the use of antibiotics, Bordeaux mixtures, and fixed coppers. g. Plant breeding has produced many plant varieties that are resistant to bacterial infection. h. Examples of bacterial plant diseases are crown gall, fireblight, walnut blight, deep phloem canker of walnuts, soft rots of vegetables, and bacterial wilt of cucumbers. 2. Fungi are small, usually microscopic, plants that lack chlorophyll and conductive tissues. a. Unlike green plants, they do not photosynthesize their own food, so they depend on living or dead plant or animal tissue. b. Only about 8,000 fungi species are known to cause plant diseases of the 100,000 species on earth. c. All plants can be attacked by some type of fungi. d. Each of the parasitic fungi can attack one or many kinds of plants. e. Some fungi grow and multiply by living on their host plant during their entire life. f. Other fungi can multiply on dead organic matter as well as living on plants. g. Fungi reproduce mainly by means of spores. 1) These spores are special reproductive bodies made up of one or a few cells. 2) The spores perform the same job as seeds in higher plants. 3) Some fungi produce up to 5 types of spores to complete a single life cycle. 4) Dissemination of fungi can be by wind, rain, insects, irrigation or flooding, contaminated seed, infected plants, animals, tillage equipment and pruning shears and knives. 5) A few have motile spores and others can grow to neighboring plants by their hyphae (thread-like strands). h. The vegetative body (Mycellium) of fungi is made up of very small filaments or threads called hyphae. 1) These branch and grow in all directions through their food supply. 2) They absorb food from the cells of their host plants. i. Fungi are controlled by several methods. 1) Chemical sprays (fungicides) have been used with success for many years. 2) Soil pasteurization and the use of fumigants works well in some instances. 3) The development of resistant species and cultivars continues to show success and more promise. 4) Crop rotation, good soil drainage, proper handling of the crop, and low temperature storage are all helpful in controlling various fungi. j. Examples of fungus diseases are stem rust of wheat, corn smut, powdery mildews, rusts, brown rot, damping off, and Dutch Elm disease. 3. Viruses are pathogenic particles that infect most higher plants and animals. a. Virus particles are extremely small (20 to 250 millimicrons). b. Viruses are not cells, nor do they consist of cells. c. They can be seen only with an electron microscope. d. They cannot grow or multiply except when they are within a host cell or insect vector cell. e. These particles move from one plant to another by vectors (carriers). 1) Most commonly aphids, leafhoppers, thrips, or mites are the vectors. 2) Also virus is spread to new plants when any asexual method is used for propagation (once virus is in the mother plant, all plants started from it will likely have virus). NOTE: Virus free propagation materials are available from the Universities and other research organizations for most major crop plants that are asexually propagated. f. Viruses can often be identified by their symptoms: 1) mosaic patterns on leaves, 2) yellows (leaves lacking chlorophyll), 3) stunting, 4) ringspots, 5) flower break, and 6) vein clearing (veins are chlorotic). g. These symptoms may be accompanied by other symptoms elsewhere on the same plant. h. The best way to control a virus is to keep it out of an area through quarantine, inspection, and certification programs. i. Other methods of control include: 1) use virus free tubers, budwood, scionwood and other propagating materials, 2) breeding resistant varieties, 3) eradication of diseased plants, 4) controlling insect vectors is rarely effective, and 5) removal of weeds which serve as virus hosts. j. As yet, no chemical substances (viricides) are available for controlling virus diseases. k. Examples of virus diseases are Tobacco mosaic, Curly Top of Sugar Beets, Barley Yellow Dwarf, Necrotic Ring Spot of Stone Fruit, Tristeza Disease of Citrus, and Blackline of Walnuts. 4. Nematodes are plant parasites belonging to the animal kingdom that are studied in plant pathology. a. Nematodes are small round worms (1/64" to 1/8"). b. They are, in general, eel-shaped, round in crossection, with smooth unsegmented bodies. c. They have no legs or other appendages. d. Plant parasitic nematodes characteristically have a stylet (spear) as a feeding apparatus. e. Their method of feeding is to puncture the cell with their stylet, secrete fluids (saliva) into the cell, then withdraw the cell contents. f. They are spread by any way that soil is moved (e.g., farm equipment, water, animals, wind, nursery plants). Major damage to plants occurs from nematodes feeding on roots. g. The above ground symptoms shown by a plant infected with nematodes is not very specific. 1. Reduced growth, nutrient deficient leaves (yellow), excess wilting are symptoms. 2. Reduced yields and poor quality products are the results. h. Below ground symptoms are more distinctive; these include the following: 1. Galls produced on roots and tubers, 2. generally a lack of feeder roots, 3. root lesions, 4. injured root tips, and 5. excessive root branching (sprangling). i. Control measures are very seldom complete, usually only reducing populations. j. Controls that are successfully used include: 1) the use of fumigants, 2) use of resistant varieties, 3) crop rotation to non host plants, and 4) summer fallow. k. At present all chemicals must be used pre-plant; none that are available for use today can be used after the crop is planted (post-plant). l. Most commonly found nematodes include Root-Knot, Root Lesion, Cyst, and Burrowing Nematode: NEMATODES COMMON NAME SCIENTIFIC NAME PRINCIPAL HOST Cyst Nematodes Heterodera small grains, corn, (nematodes that form carrot, crucifers cysts covering the eggs) soybean, Pea, hop wheat, Irish potato tomato, eggplant, legumes Root Knot Nematodes Meloidogyne 1700 hosts including Forage, small grains, grasses, fruit, vegetables, field crops,nursery, weeds Root Lesion Nematode Pratylenchus Potato, corn, peanut, cotton, pineapple, avocado, tobacco, olive, apple, wheat, oats, strawberry, tomato, lily, walnut, grape, fig, citrus, rose Burrowing Nematode Radopholus Citrus, ornamentals, avocados D. Nutrition affects the rate of growth and the state of readiness of plants to defend themselves against attack by pathogens. 1. High nitrogen fertilization increases the susceptibility of some plants to bacterial and fungal diseases. 2. Proper nutrition of phosphorous, potassium, calcium, and micronutrients have been shown to aid a plants resistance to certain diseases. 3. In general, plants receiving a balanced nutrition, are more capable of protecting themselves than plants with either excessive or deficient amounts of nutrients. __________________________________________________________ ACTIVITY: 1. Rot soft fruits in class (e.g. peaches, cherries, plums). a. Leave some fruit intact. b. Injure others to simulate insect or bird injury. c. Observe and record results. 2. Visit local fields, orchards, or vineyards infected with plant disease pathogens. a. Compare results of different pest management programs. b. Was the cost of the program warranted? __________________________________________________________ 7/24/90 sg #%&C